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Immunohistochemical staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.

Anti-CDK16 Antibody

HPA001366
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCDK16
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CDK16
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5127
  • Target name
    CDK16
  • Target description
    cyclin dependent kinase 16
  • Target synonyms
    PCTAIRE, PCTAIRE1, PCTGAIRE, PCTK1, cyclin-dependent kinase 16, PCTAIRE-motif protein kinase 1, cell division protein kinase 16, serine/threonine-protein kinase PCTAIRE-1, testis secretory sperm-binding protein Li 224n
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ00536
  • Protein Name
    Cyclin-dependent kinase 16
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of CDK16 using anti-CDK16 antibody (A06102-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK16 antigen affinity purified polyclonal antibody (Catalog # A06102-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK16 at approximately 56KD. The expected band size for CDK16 is at 56KD.
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