Anti-Collagen type II [CIIC1 ]

Absolute Antibody

Anti-Collagen type II [CIIC1 ]

9

The antibody binds the triple helical C1 epitope (GARGLT) of collagen type II.

This anti-collagen type II antibody CIIC1 is an arthritogenic antibody, which induces arthritis in mice (Holmdahl et al., 1986; PMID: 2421741 and Nandakumar et al., 2003; PMID: 12884302). The antibody specificity towards collagen type II was assayed in an ELISA. The antibody was selected for in vivo injection experiments in naive DBA/1 male mice, at no time was any arthritis detected macroscopically after treatment with 0.5, 2 or 5 mg of antibody per animal, however development of synovitis was noticed (Holmdahl et al., 1986; PMID: 2421741). The structure of the Fab fragment was determined by X-ray crystallography. The specificity of the full length CIIC1 antibody and the FV fragment was confirmed by ELISA analysis. Further investigation by using both the full length antibody and a single chain Fv fragment in ELISA experiments revealed that the antibody bound also to other IgG1, IgG2a and IgG2b anti-collagen antibodies (Uysal et al., 2008; PMID: 18241923). The antibody detected collagene type II by western blot analysis (Burkhardt et al., 2005; PMID: 15832289). In vitro studies demonstrated that the antibody together with M2139 antibody suppressed the self-assembly of CII into fibrils (Croxford et al., 2010; PMID: 20662051). This antibody was used for immunohistochemistry on cartilaginous tissue (Vickers et al., 2010; PMID: 20225321).

Western Blot, ELISA, ImmunoHistoChemistry, Other Application

Research Use Only

Monoclonal

CIIC1

COL2A1

collagen type II alpha 1 chain

alpha-1 type II collagen; ANFH; AOM; arthroophthalmopathy, progressive (Stickler syndrome); cartilage collagen; chondrocalcin; COL11A3; collagen alpha-1(II) chain; collagen II, alpha-1 polypeptide; collagen, type II, alpha 1; SEDC; STL1

Human

IgG1

Collagen alpha-1(II) chain

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.

Bovine, Chicken, Human, Mouse, Rat

-20°C,2°C to 8°C

12352203