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anti-CYFIP1 antibody

ARG42817
Arigo Biolaboratories
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetCYFIP1
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Overview

  • Supplier
    Arigo Biolaboratories
  • Product Name
    anti-CYFIP1 antibody
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23191
  • Target name
    CYFIP1
  • Target description
    cytoplasmic FMR1 interacting protein 1
  • Target synonyms
    cytoplasmic FMR1-interacting protein 1; cytoplasmic FMRP interacting protein 1; P140SRA-1; selective hybridizing clone; SHYC; specifically Rac1-associated protein 1; SRA1; SRA-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    This gene encodes a protein that regulates cytoskeletal dynamics and protein translation. The encoded protein is a component of the WAVE regulatory complex (WRC), which promotes actin polymerization.This protein also interacts with the Fragile X mental retardation protein (FMRP) and translation initiation factor 4E to inhibit protein translation. A large chromosomal deletion including this gene is associated with increased risk of schizophrenia and epilepsy in human patients. Reduced expression of this gene has been observed in various human cancers and the encoded protein may inhibit tumor invasion. [provided by RefSeq, May 2017]
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CYFIP1 using anti-CYFIP1 antibody (M04596). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYFIP1 antigen affinity purified monoclonal antibody (Catalog # M04596) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYFIP1 at approximately 145 kDa. The expected band size for CYFIP1 is at 145 kDa.
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