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Immunohistochemical staining of human caudate shows strong cytoplasmic and nucleolar positivity in neuronal cells.
Immunohistochemical staining of human caudate shows strong cytoplasmic and nucleolar positivity in neuronal cells.
Immunohistochemical staining of human caudate shows strong cytoplasmic and nucleolar positivity in neuronal cells.

Anti-CYFIP1 Antibody

HPA068106
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetCYFIP1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CYFIP1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23191
  • Target name
    CYFIP1
  • Target description
    cytoplasmic FMR1 interacting protein 1
  • Target synonyms
    cytoplasmic FMR1-interacting protein 1; cytoplasmic FMRP interacting protein 1; P140SRA-1; selective hybridizing clone; SHYC; specifically Rac1-associated protein 1; SRA1; SRA-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7L576
  • Protein Name
    Cytoplasmic FMR1-interacting protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CYFIP1 using anti-CYFIP1 antibody (M04596). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYFIP1 antigen affinity purified monoclonal antibody (Catalog # M04596) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYFIP1 at approximately 145 kDa. The expected band size for CYFIP1 is at 145 kDa.
Product group Antibodies
Boster Bio
Anti-CYFIP1 Rabbit Monoclonal AntibodyM04596
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetCYFIP1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
CYFIP1 AntibodyLS-C496999
ApplicationsWestern Blot
TargetCYFIP1
  • SizePrice
Product group Antibodies
RayBiotech
Anti-CYFIP1 Antibody144-10291
ApplicationsWestern Blot
TargetCYFIP1
  • SizePrice
Product group Antibodies
Bethyl Laboratories
Rabbit anti-CYFIP1 Antibody, Affinity PurifiedA302-996A
ApplicationsImmunoPrecipitation, Western Blot
TargetCYFIP1
  • SizePrice
Product group Antibodies
Bioss Antibodies
Cytoplasmic FMR1 interacting protein 1 Recombinant Antbody Kit (KD-Validated)BSM-90217R
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry
TargetCYFIP1
  • SizePrice
Product group Antibodies
Assay Genie
CYFIP1 AntibodyCAB10291
TargetCYFIP1
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Immunofluorescence staining of A549 cells with CSB-PA748722LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Product group Antibodies
Cusabio
CYFIP1 AntibodyCSB-PA748722LA01HU
ApplicationsImmunoFluorescence, ELISA
ReactivityHuman
TargetCYFIP1
  • SizePrice
Product group Antibodies
References
GeneTex
CYFIP1 antibody [N1N2], N-termGTX122467
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetCYFIP1
  • SizePrice
Anti-CYFIP1 Antibody
Product group Antibodies
Antibodies.com
Anti-CYFIP1 AntibodyA28942
ApplicationsWestern Blot
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Product group Antibodies
MedChem Express
CYFIP1 Antibody (YA2605, Rabbit)HY-P82860
TargetCYFIP1
  • SizePrice