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CYFIP1 Antibody

CAB10291
Assay Genie
TargetCYFIP1
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    CYFIP1 Rabbit pAb
  • Delivery Days Customer
    9
  • Applications Supplier
    WB,IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID23191
  • Target name
    CYFIP1
  • Target description
    cytoplasmic FMR1 interacting protein 1
  • Target synonyms
    cytoplasmic FMR1-interacting protein 1; cytoplasmic FMRP interacting protein 1; P140SRA-1; selective hybridizing clone; SHYC; specifically Rac1-associated protein 1; SRA1; SRA-1
  • Host
    Rabbit
  • Protein IDQ7L576
  • Protein Name
    Cytoplasmic FMR1-interacting protein 1
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CYFIP1 using anti-CYFIP1 antibody (M04596). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYFIP1 antigen affinity purified monoclonal antibody (Catalog # M04596) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYFIP1 at approximately 145 kDa. The expected band size for CYFIP1 is at 145 kDa.
Product group Antibodies
Boster Bio
Anti-CYFIP1 Rabbit Monoclonal AntibodyM04596
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetCYFIP1
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Product group Antibodies
LifeSpan BioSciences
CYFIP1 AntibodyLS-C496999
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RayBiotech
Anti-CYFIP1 Antibody144-10291
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Bethyl Laboratories
Rabbit anti-CYFIP1 Antibody, Affinity PurifiedA302-996A
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Bioss Antibodies
Cytoplasmic FMR1 interacting protein 1 Recombinant Antbody Kit (KD-Validated)BSM-90217R
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Immunofluorescence staining of A549 cells with CSB-PA748722LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Product group Antibodies
Cusabio
CYFIP1 AntibodyCSB-PA748722LA01HU
ApplicationsImmunoFluorescence, ELISA
ReactivityHuman
TargetCYFIP1
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Immunohistochemical staining of human caudate shows strong cytoplasmic and nucleolar positivity in neuronal cells.
Product group Antibodies
Atlas Antibodies
Anti-CYFIP1 AntibodyHPA068106
ApplicationsImmunoHistoChemistry
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TargetCYFIP1
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Product group Antibodies
References
GeneTex
CYFIP1 antibody [N1N2], N-termGTX122467
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetCYFIP1
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Antibodies.com
Anti-CYFIP1 AntibodyA28942
ApplicationsWestern Blot
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Product group Antibodies
MedChem Express
CYFIP1 Antibody (YA2605, Rabbit)HY-P82860
TargetCYFIP1
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