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Anti-Cytokeratin 7 Antibody

188-10504
RayBiotech
ApplicationsFlow Cytometry
Product group Antibodies
TargetKRT7
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-Cytokeratin 7 Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Concentration
    0.2 mg/ml
  • Gene ID3855
  • Target name
    KRT7
  • Target description
    keratin 7
  • Target synonyms
    CK7; CK-7; cytokeratin 7; K2C7; K7; keratin 7, type II; keratin, 55K type II cytoskeletal; keratin, simple epithelial type I, K7; keratin, type II cytoskeletal 7; sarcolectin; SCL; type II mesothelial keratin K7; type-II keratin Kb7
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP08729
  • Protein Name
    Keratin, type II cytoskeletal 7
  • Scientific Description
    Mouse anti-Human Cytokeratin 7 Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KRT7 using anti-KRT7 antibody (A02416-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KRT7 antigen affinity purified polyclonal antibody (Catalog # A02416-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KRT7 at approximately 51 kDa. The expected band size for KRT7 is at 51 kDa.
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1) Input: Hela Cell Lysate 2) IP product: IP dilute 1:200
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