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ProductsBack/Cytokeratin 7 antibody [N1C2]

Cytokeratin 7 antibody [N1C2]

GTX109723
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetKRT7
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Overview

  • Supplier
    GeneTex
  • Product Name
    Cytokeratin 7 antibody [N1C2]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.76 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3855
  • Target name
    KRT7
  • Target description
    keratin 7
  • Target synonyms
    CK7; CK-7; cytokeratin 7; K2C7; K7; keratin 7, type II; keratin, 55K type II cytoskeletal; keratin, simple epithelial type I, K7; keratin, type II cytoskeletal 7; sarcolectin; SCL; type II mesothelial keratin K7; type-II keratin Kb7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08729
  • Protein Name
    Keratin, type II cytoskeletal 7
  • Scientific Description
    The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • FOXM1-CD44 Signaling Is Critical for the Acquisition of Regorafenib Resistance in Human Liver Cancer Cells.
    Read more
  • Visfatin Enhances Breast Cancer Progression through CXCL1 Induction in Tumor-Associated Macrophages. Wang YY et al., 2020 Nov 26, Cancers (Basel)
    Read more
  • Improving the reproducibility, accuracy, and stability of an electrochemical biosensor platform for point-of-care use. Chen LC et al., 2020 May 1, Biosens Bioelectron
    Read more
  • ZEB2, a master regulator of the epithelial-mesenchymal transition, mediates trophoblast differentiation. DaSilva-Arnold SC et al., 2019 Feb 1, Mol Hum Reprod
    Read more
  • Human trophoblast epithelial-mesenchymal transition in abnormally invasive placenta. DaSilva-Arnold SC et al., 2018 Aug 1, Biol Reprod
    Read more
  • TOP2A induces malignant character of pancreatic cancer through activating beta-catenin signaling pathway. Pei YF et al., 2018 Jan, Biochim Biophys Acta Mol Basis Dis
    Read more
  • Endoglin Is Essential for the Maintenance of Self-Renewal and Chemoresistance in Renal Cancer Stem Cells. Hu J et al., 2017 Aug 8, Stem Cell Reports
    Read more

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Figure 1. Western blot analysis of KRT7 using anti-KRT7 antibody (A02416-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KRT7 antigen affinity purified polyclonal antibody (Catalog # A02416-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KRT7 at approximately 51 kDa. The expected band size for KRT7 is at 51 kDa.
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