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ProductsBack/Cytokeratin 7 antibody [N1C2]

Cytokeratin 7 antibody [N1C2]

GTX109723
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetKRT7
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Overview

  • Supplier
    GeneTex
  • Product Name
    Cytokeratin 7 antibody [N1C2]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.76 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3855
  • Target name
    KRT7
  • Target description
    keratin 7
  • Target synonyms
    CK7, K2C7, K7, SCL, keratin, type II cytoskeletal 7, CK-7, cytokeratin 7, keratin 7, type II, keratin, 55K type II cytoskeletal, keratin, simple epithelial type I, K7, sarcolectin, type II mesothelial keratin K7, type-II keratin Kb7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08729
  • Protein Name
    Keratin, type II cytoskeletal 7
  • Scientific Description
    The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Wuputra K, Hsiao PJ, Chang WT, et al. FOXM1-CD44 Signaling Is Critical for the Acquisition of Regorafenib Resistance in Human Liver Cancer Cells. Int J Mol Sci. 2022,23(14). doi: 10.3390/ijms23147782
    Read this paper
  • Wang YY, Chen HD, Lo S, et al. Visfatin Enhances Breast Cancer Progression through CXCL1 Induction in Tumor-Associated Macrophages. Cancers (Basel). 2020,12(12). doi: 10.3390/cancers12123526
    Read this paper
  • Chen LC, Wang E, Tai CS, et al. Improving the reproducibility, accuracy, and stability of an electrochemical biosensor platform for point-of-care use. Biosens Bioelectron. 2020,155:112111. doi: 10.1016/j.bios.2020.112111
    Read this paper
  • DaSilva-Arnold SC, Kuo CY, Davra V, et al. ZEB2, a master regulator of the epithelial-mesenchymal transition, mediates trophoblast differentiation. Mol Hum Reprod. 2019,25(2):61-75. doi: 10.1093/molehr/gay053
    Read this paper
  • DaSilva-Arnold SC, Zamudio S, Al-Khan A, et al. Human trophoblast epithelial-mesenchymal transition in abnormally invasive placenta. Biol Reprod. 2018,99(2):409-421. doi: 10.1093/biolre/ioy042
    Read this paper
  • Pei YF, Yin XM, Liu XQ. TOP2A induces malignant character of pancreatic cancer through activating β-catenin signaling pathway. Biochim Biophys Acta Mol Basis Dis. 2018,1864(1):197-207. doi: 10.1016/j.bbadis.2017.10.019
    Read this paper
  • Hu J, Guan W, Liu P, et al. Endoglin Is Essential for the Maintenance of Self-Renewal and Chemoresistance in Renal Cancer Stem Cells. Stem Cell Reports. 2017,9(2):464-477. doi: 10.1016/j.stemcr.2017.07.009
    Read this paper

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Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CK7 Polyclonal Antibody(bs-1610R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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Figure 1. Western blot analysis of KRT7 using anti-KRT7 antibody (A02416-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KRT7 antigen affinity purified polyclonal antibody (Catalog # A02416-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KRT7 at approximately 51 kDa. The expected band size for KRT7 is at 51 kDa.
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