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Cytokeratin 7 Antibody (YA950, Mouse)

HY-P81267
MedChem Express
TargetKRT7
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Cytokeratin 7 Antibody (YA950, Mouse)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID3855
  • Target name
    KRT7
  • Target description
    keratin 7
  • Target synonyms
    CK7; CK-7; cytokeratin 7; K2C7; K7; keratin 7, type II; keratin, 55K type II cytoskeletal; keratin, simple epithelial type I, K7; keratin, type II cytoskeletal 7; sarcolectin; SCL; type II mesothelial keratin K7; type-II keratin Kb7
  • Host
    Mouse
  • Isotype
    IgG2b
  • Scientific Description
    Cytokeratin 7 Antibody (YA950) is a mouse-derived non-conjugated IgG2b antibody (Clone NO.: YA950), targeting Cytokeratin 7, with a predicted molecular weight of 51 kDa (observed band size: 55 kDa). Cytokeratin 7 Antibody (YA950) can be used for WB, ICC/IF experiment in human background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of KRT7 using anti-KRT7 antibody (A02416-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KRT7 antigen affinity purified polyclonal antibody (Catalog # A02416-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KRT7 at approximately 51 kDa. The expected band size for KRT7 is at 51 kDa.
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1) Input: Hela Cell Lysate 2) IP product: IP dilute 1:200
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