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Immunohistochemical staining of human salivary gland shows cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human salivary gland shows cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human salivary gland shows cytoplasmic and membranous positivity in glandular cells.

Anti-DCTN1 Antibody

HPA069977
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadMSDS
Product group Antibodies
TargetDCTN1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-DCTN1
  • Delivery Days Customer
    12
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1639
  • Target name
    DCTN1
  • Target description
    dynactin subunit 1
  • Target synonyms
    150 kDa dynein-associated polypeptide; DAP-150; DP-150; dynactin 1 (p150, glued homolog, Drosophila); dynactin subunit 1; P135
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14203
  • Protein Name
    Dynactin subunit 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DCTN1/p150-glued using anti-DCTN1/p150-glued antibody (A02175-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human placenta tissue lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: human Caco-2 whole cell lysates, Lane 6: human U20S whole cell lysates, Lane 7: human HEK293 whole cell lysates, Lane 8: human HepG2 whole cell lysates, Lane 9: rat brain tissue lysates, Lane 10: rat stomach tissue lysates, Lane 11: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DCTN1/p150-glued antigen affinity purified polyclonal antibody (Catalog # A02175-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DCTN1/p150-glued at approximately 150KD. The expected band size for DCTN1/p150-glued is at 150KD.
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