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ProductsBack/Goat anti-Dynactin

Goat anti-Dynactin

EB05639
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetDCTN1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-Dynactin Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells and additionally nucleus of U2OS cells. Recommended concentration: 10microg/ml. Immunocytochemsitry: A customer found positive results in ICC on Mouse embryonic fibroblasts (MEF cells). Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, FC, IF, ICC, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID1639
  • Target name
    DCTN1
  • Target description
    dynactin subunit 1
  • Target synonyms
    150 kDa dynein-associated polypeptide; DAP-150; DP-150; dynactin 1 (p150, glued homolog, Drosophila); dynactin subunit 1; P135
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_004073.2; NP_075408.1; NP_001128512.1; NP_001128513.1; NP_001177765.1; NP_001177766.1. GeneIDs all Nonhuman: 13191 (mouse);. Purification: Antigen affinity purified. Names and symbols: DCTN1; dynactin 1 (p150, glued homolog, Drosophila); P135; DP-150; DAP-150; p150-glued; p150, Glued (Drosophila) homolog; 150 kDa dynein-associated polypeptide; dynactin 1 (p150, Glued (Drosophila) homolog); HMN7B; OTTHUMP00000202491; dynactin 1; p150, Gl
  • Reactivity Supplier
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DCTN1/p150-glued using anti-DCTN1/p150-glued antibody (A02175-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human placenta tissue lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: human Caco-2 whole cell lysates, Lane 6: human U20S whole cell lysates, Lane 7: human HEK293 whole cell lysates, Lane 8: human HepG2 whole cell lysates, Lane 9: rat brain tissue lysates, Lane 10: rat stomach tissue lysates, Lane 11: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DCTN1/p150-glued antigen affinity purified polyclonal antibody (Catalog # A02175-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DCTN1/p150-glued at approximately 150KD. The expected band size for DCTN1/p150-glued is at 150KD.
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