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Anti-Dystrophin [130D2-1], Human IgG1, kappa

AB04680-10.0
Absolute Antibody
ReactivityHuman
Product group Antibodies
TargetDMD
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Dystrophin [130D2-1], Human IgG1, kappa
  • Delivery Days Customer
    9
  • Application Supplier Note
    The original antibody was used for LBA/LCMS on beagle skeletal muscle and human skeletal muscle to quantify microdystrophin. The binding affinity of this antibody was assessed using an MSD assay on the LEM peptide it showed good affinity for its peptide (WO2023064947A1).
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    130D2-1
  • Gene ID1756
  • Target name
    DMD
  • Target description
    dystrophin
  • Target synonyms
    BMD, CMD3B, DXS142, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272, MRX85, dystrophin, mutant dystrophin
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDP11532
  • Protein Name
    Dystrophin
  • Scientific Description
    This chimeric human antibody was made using the variable domain sequences of the original Rabbit IgG format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Dystrophin using anti-Dystrophin antibody (PB9276). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: mouse HEPA1-6 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dystrophin antigen affinity purified polyclonal antibody (Catalog # PB9276) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dystrophin at approximately 427KD. The expected band size for Dystrophin is at 427KD.
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