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Anti-EME1 Antibody

Anti-EME1 Antibody

A17234
Antibodies.com
ApplicationsWestern Blot
Product group Antibodies
ReactivityMouse, Rat
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-EME1 Antibody
  • Delivery Days Customer
    7
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Rabbit polyclonal antibody to EME1.
  • Reactivity
    Mouse, Rat
  • UNSPSC
    12352203

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Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EME1 antibody [N3C2], Internal (GTX100132) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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Figure 1. Western blot analysis of EME1 using anti-EME1 antibody (PB9804). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HELA Whole Cell Lysate, Lane 2: JURKAT Whole Cell Lysate, Lane 3: HUT Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EME1 antigen affinity purified polyclonal antibody (Catalog # PB9804) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EME1 at approximately 62KD. The expected band size for EME1 is at 62KD.
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