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ProductsBack/EME1 antibody [N3C2], Internal
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EME1 antibody [N3C2], Internal (GTX100132) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EME1 antibody [N3C2], Internal (GTX100132) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EME1 antibody [N3C2], Internal (GTX100132) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

EME1 antibody [N3C2], Internal

GTX100132
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetEME1
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Overview

  • Supplier
    GeneTex
  • Product Name
    EME1 antibody [N3C2], Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID146956
  • Target name
    EME1
  • Target description
    essential meiotic structure-specific endonuclease 1
  • Target synonyms
    MMS4L, SLX2A, structure-specific endonuclease subunit EME1, MMS4 homolog, SLX2 structure-specific endonuclease subunit homolog A, crossover junction endonuclease EME1, essential meiotic endonuclease 1 homolog 1, essential meiotic endonuclease 1 homolog 2, hMMS4, homolog of yeast EME1 endonuclease
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96AY2
  • Protein Name
    Structure-specific endonuclease subunit EME1
  • Scientific Description
    EME1 and MUS81 (MIM 606591) form an endonuclease complex that cleaves branched DNA structures, especially those arising during stalled DNA replication (Abraham et al., 2003 [PubMed 14609959]).[supplied by OMIM]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of EME1 using anti-EME1 antibody (PB9804). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HELA Whole Cell Lysate, Lane 2: JURKAT Whole Cell Lysate, Lane 3: HUT Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EME1 antigen affinity purified polyclonal antibody (Catalog # PB9804) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EME1 at approximately 62KD. The expected band size for EME1 is at 62KD.
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