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EME1 Polyclonal Antibody

BS-7861R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
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Product group Antibodies
TargetEME1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    EME1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID146956
  • Target name
    EME1
  • Target description
    essential meiotic structure-specific endonuclease 1
  • Target synonyms
    crossover junction endonuclease EME1; essential meiotic endonuclease 1 homolog 1; essential meiotic endonuclease 1 homolog 2; hMMS4; homolog of yeast EME1 endonuclease; MMS4 homolog; MMS4L; SLX2 structure-specific endonuclease subunit homolog A; SLX2A
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EME1 using anti-EME1 antibody (PB9804). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HELA Whole Cell Lysate, Lane 2: JURKAT Whole Cell Lysate, Lane 3: HUT Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EME1 antigen affinity purified polyclonal antibody (Catalog # PB9804) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EME1 at approximately 62KD. The expected band size for EME1 is at 62KD.
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