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Western Blot analysis of COLO cells using Eme1 Polyclonal Antibody
Western Blot analysis of COLO cells using Eme1 Polyclonal Antibody
Western Blot analysis of COLO cells using Eme1 Polyclonal Antibody

EME1 Antibody

CSB-PA007381
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetEME1
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Overview

  • Supplier
    Cusabio
  • Product Name
    EME1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID146956
  • Target name
    EME1
  • Target description
    essential meiotic structure-specific endonuclease 1
  • Target synonyms
    crossover junction endonuclease EME1; essential meiotic endonuclease 1 homolog 1; essential meiotic endonuclease 1 homolog 2; hMMS4; homolog of yeast EME1 endonuclease; MMS4 homolog; MMS4L; SLX2 structure-specific endonuclease subunit homolog A; SLX2A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96AY2
  • Protein Name
    Crossover junction endonuclease EME1
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EME1 using anti-EME1 antibody (PB9804). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HELA Whole Cell Lysate, Lane 2: JURKAT Whole Cell Lysate, Lane 3: HUT Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EME1 antigen affinity purified polyclonal antibody (Catalog # PB9804) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EME1 at approximately 62KD. The expected band size for EME1 is at 62KD.
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