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Anti-ERCC2 Antibody

144-60710
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetERCC2
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-ERCC2 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2068
  • Target name
    ERCC2
  • Target description
    ERCC excision repair 2, TFIIH core complex helicase subunit
  • Target synonyms
    COFS2, CXPD, EM9, TFIIH, TTD, TTD1, XPD, general transcription and DNA repair factor IIH helicase subunit XPD, BTF2 p80, DNA 5'-3' helicase XPD, DNA excision repair protein ERCC-2, DNA repair protein complementing XP-D cells, TFIIH 80 kDa subunit, TFIIH basal transcription factor complex 80 kDa subunit, TFIIH basal transcription factor complex helicase XPD subunit, TFIIH p80, TFIIH subunit XPD, basic transcription factor 2 80 kDa subunit, excision repair cross-complementation group 2, excision repair cross-complementing rodent repair deficiency, complementation group 2, xeroderma pigmentosum complementary group D, xeroderma pigmentosum group D-complementing protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP18074
  • Protein Name
    General transcription and DNA repair factor IIH helicase subunit XPD
  • Scientific Description
    ERCC2 Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of XPD/ERCC2 using anti-XPD/ERCC2 antibody (A00694-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XPD/ERCC2 antigen affinity purified polyclonal antibody (Catalog # A00694-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XPD/ERCC2 at approximately 87 kDa. The expected band size for XPD/ERCC2 is at 80 kDa.
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