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ProductsBack/XPD Antibody (YA2434, Rabbit)

XPD Antibody (YA2434, Rabbit)

HY-P82689
MedChem Express
TargetERCC2
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    XPD Antibody (YA2434, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID2068
  • Target name
    ERCC2
  • Target description
    ERCC excision repair 2, TFIIH core complex helicase subunit
  • Target synonyms
    COFS2, CXPD, EM9, TFIIH, TTD, TTD1, XPD, general transcription and DNA repair factor IIH helicase subunit XPD, BTF2 p80, DNA 5'-3' helicase XPD, DNA excision repair protein ERCC-2, DNA repair protein complementing XP-D cells, TFIIH 80 kDa subunit, TFIIH basal transcription factor complex 80 kDa subunit, TFIIH basal transcription factor complex helicase XPD subunit, TFIIH p80, TFIIH subunit XPD, basic transcription factor 2 80 kDa subunit, excision repair cross-complementation group 2, excision repair cross-complementing rodent repair deficiency, complementation group 2, xeroderma pigmentosum complementary group D, xeroderma pigmentosum group D-complementing protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    XPD Antibody (YA2434) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2434), targeting XPD, with a predicted molecular weight of 87 kDa (observed band size: 80 kDa). XPD Antibody (YA2434) can be used for WB, ICC/IF experiment in human background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of XPD/ERCC2 using anti-XPD/ERCC2 antibody (A00694-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XPD/ERCC2 antigen affinity purified polyclonal antibody (Catalog # A00694-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XPD/ERCC2 at approximately 87 kDa. The expected band size for XPD/ERCC2 is at 80 kDa.
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