Figure 1. Western blot analysis of EWSR1 using anti-EWSR1 antibody (M00589). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: K562 whole cell lysates, Lane 2: Jurkat whole cell lysates, Lane 3: A549 whole cell lysates, Lane 4: MCF-7 whole cell lysates, Lane 5: COLO-320 whole cell lysates, Lane 6: SW620 whole cell lysates, Lane 7: A431 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-EWSR1 antigen affinity purified monoclonal antibody (Catalog # M00589) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for EWSR1 at approximately 95KD. The expected band size for EWSR1 is at 95KD.