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Immunohistochemical staining of human bronchus shows cilia positivity in respiratory epithelial cells.
Immunohistochemical staining of human bronchus shows cilia positivity in respiratory epithelial cells.
Immunohistochemical staining of human bronchus shows cilia positivity in respiratory epithelial cells.

Anti-FLI1 Antibody

HPA065030
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetFLI1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-FLI1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2313
  • Target name
    FLI1
  • Target description
    Fli-1 proto-oncogene, ETS transcription factor
  • Target synonyms
    BDPLT21; Ewing sarcoma breakpoint region 2; EWSR2; Friend leukemia integration 1 transcription factor; Friend leukemia virus integration 1; SIC-1; transcription factor ERGB
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ01543
  • Protein Name
    Friend leukemia integration 1 transcription factor
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FLI1 using anti-FLI1 antibody (A00399). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse lung tissue lysates, Lane 6: mouse RAW246.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FLI1 antigen affinity purified polyclonal antibody (Catalog # A00399) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FLI1 at approximately 54KD. The expected band size for FLI1 is at 51KD.
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