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ProductsBack/Anti-FoxO4 (P191) Antibody

Anti-FoxO4 (P191) Antibody

A26999

ApplicationsWestern Blot, ImmunoHistoChemistry

Product group Antibodies

Overview

Supplier
Antibodies.com
Product Name
Anti-FoxO4 (P191) Antibody
Delivery Days Customer
7
Applications
Western Blot, ImmunoHistoChemistry
Certification
Research Use Only
Clonality
Polyclonal
Conjugate
Unconjugated
Estimated Purity
>95%
Host
Rabbit
Scientific Description
Rabbit polyclonal antibody to FoxO4 (P191)
UNSPSC
12352203

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Figure 1. Western blot analysis of FOXO4 using anti-FOXO4 antibody (M01819-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat skeletal muscle tissue lysates, Lane 6: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXO4 antigen affinity purified monoclonal antibody (M01819-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXO4 at approximately 65 kDa. The expected band size for FOXO4 is at 54 kDa.

Product group Antibodies

Anti-FoxO4/Afx Rabbit Monoclonal AntibodyM01819-1

ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry

TargetFOXO4

SizePrice

Product group Antibodies

phospho-FOXO4 (Ser262) Polyclonal AntibodyBS-3145R

ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

TargetFOXO4