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Western Blot analysis of COS7 cells using FoxO4 Polyclonal Antibody
Western Blot analysis of COS7 cells using FoxO4 Polyclonal Antibody
Western Blot analysis of COS7 cells using FoxO4 Polyclonal Antibody

FOXO4 Antibody

CSB-PA002575
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Monkey, Mouse
TargetFOXO4
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Overview

  • Supplier
    Cusabio
  • Product Name
    FOXO4 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4303
  • Target name
    FOXO4
  • Target description
    forkhead box O4
  • Target synonyms
    AFX; AFX1; fork head domain transcription factor AFX1; forkhead box protein O4; MLLT7; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP98177
  • Protein Name
    Forkhead box protein O4
  • Reactivity
    Human, Monkey, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FOXO4 using anti-FOXO4 antibody (M01819-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat skeletal muscle tissue lysates, Lane 6: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXO4 antigen affinity purified monoclonal antibody (M01819-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXO4 at approximately 65 kDa. The expected band size for FOXO4 is at 54 kDa.
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