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ProductsBack/Anti-Phospho-FOXO4-S197 Antibody

Anti-Phospho-FOXO4-S197 Antibody

144-50660
RayBiotech
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetFOXO4
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-Phospho-FOXO4-S197 Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Gene ID4303
  • Target name
    FOXO4
  • Target description
    forkhead box O4
  • Target synonyms
    AFX; AFX1; fork head domain transcription factor AFX1; forkhead box protein O4; MLLT7; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP98177
  • Protein Name
    Forkhead box protein O4
  • Scientific Description
    Phospho-FOXO4-S197 pAb
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FOXO4 using anti-FOXO4 antibody (M01819-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat skeletal muscle tissue lysates, Lane 6: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXO4 antigen affinity purified monoclonal antibody (M01819-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXO4 at approximately 65 kDa. The expected band size for FOXO4 is at 54 kDa.
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