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Immunohistochemical staining of human Fallopian tube shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human Fallopian tube shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human Fallopian tube shows strong cytoplasmic positivity in glandular cells.

Anti-HDDC3 Antibody

HPA040895
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetHDDC3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-HDDC3
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID374659
  • Target name
    HDDC3
  • Target description
    HD domain containing 3
  • Target synonyms
    (ppGpp)ase, MESH1, MYNRL15, guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase MESH1, HD domain-containing protein 3, metazoan SpoT homolog 1, myeloid leukemia noncoding regulatory locus on chromosome 15, penta-phosphate guanosine-3'-pyrophosphohydrolase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N4P3
  • Protein Name
    Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase MESH1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of HDDC3 using anti-HDDC3 antibody (A12287-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDDC3 antigen affinity purified polyclonal antibody (Catalog # A12287-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDDC3 at approximately 22 kDa. The expected band size for HDDC3 is at 20 kDa.
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