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HDDC3 Antibody

PACO38538
Assay Genie
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetHDDC3
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Overview

  • Supplier
    Assay Genie
  • Product Name
    HDDC3 Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    Not applicable
  • Conjugate
    Unconjugated
  • Gene ID374659
  • Target name
    HDDC3
  • Target description
    HD domain containing 3
  • Target synonyms
    (ppGpp)ase, MESH1, MYNRL15, guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase MESH1, HD domain-containing protein 3, metazoan SpoT homolog 1, myeloid leukemia noncoding regulatory locus on chromosome 15, penta-phosphate guanosine-3'-pyrophosphohydrolase
  • Host
    Rabbit
  • Protein IDQ8N4P3
  • Protein Name
    Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase MESH1
  • Scientific Description
    HDDC3 Antibody is a premium polyclonal that offers outstanding performance and reliability for demanding research applications. Rigorously validated for ELISA, WB, IHC, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human, Mouse samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 50ug format to meet your experimental needs. For optimal performance, store at -20°C or -80°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human, Mouse
  • Reactivity Supplier
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of HDDC3 using anti-HDDC3 antibody (A12287-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDDC3 antigen affinity purified polyclonal antibody (Catalog # A12287-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDDC3 at approximately 22 kDa. The expected band size for HDDC3 is at 20 kDa.
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