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Western blot All lanes: HDDC3 antibody at 2ug/ml + Mouse heart tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 21, 16 kDa Observed band size: 21 kDa
Western blot All lanes: HDDC3 antibody at 2ug/ml + Mouse heart tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 21, 16 kDa Observed band size: 21 kDa
Western blot All lanes: HDDC3 antibody at 2ug/ml + Mouse heart tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 21, 16 kDa Observed band size: 21 kDa

HDDC3 Antibody

CSB-PA836666HA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetHDDC3
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Overview

  • Supplier
    Cusabio
  • Product Name
    HDDC3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID374659
  • Target name
    HDDC3
  • Target description
    HD domain containing 3
  • Target synonyms
    (ppGpp)ase, MESH1, MYNRL15, guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase MESH1, HD domain-containing protein 3, metazoan SpoT homolog 1, myeloid leukemia noncoding regulatory locus on chromosome 15, penta-phosphate guanosine-3'-pyrophosphohydrolase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N4P3
  • Protein Name
    Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase MESH1
  • Scientific Description
    ppGpp hydrolyzing enzyme involved in starvation response.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HDDC3 using anti-HDDC3 antibody (A12287-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDDC3 antigen affinity purified polyclonal antibody (Catalog # A12287-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDDC3 at approximately 22 kDa. The expected band size for HDDC3 is at 20 kDa.
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