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Anti-HNF-4-alpha Antibody

A285970
Antibodies.com
ApplicationsImmunoFluorescence, ELISA
Product group Antibodies
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-HNF4A Antibody
  • Delivery Days Customer
    7
  • Applications
    ImmunoFluorescence, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Host
    Goat
  • Isotype
    IgG
  • Scientific Description
    Goat polyclonal antibody to HNF-4-alpha.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HNF-4-alpha using anti-HNF-4-alpha antibody (M00179-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HNF-4-alpha antigen affinity purified monoclonal antibody (Catalog # M00179-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for HNF-4-alpha at approximately 53 kDa. The expected band size for HNF-4-alpha is at 53 kDa.
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