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Goat anti-HNF4A

EB06700
Everest Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetHNF4A
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-HNF4A Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID3172
  • Target name
    HNF4A
  • Target description
    hepatocyte nuclear factor 4 alpha
  • Target synonyms
    FRTS4; hepatic nuclear factor 4 alpha; hepatocyte nuclear factor 4-alpha; HNF4; HNF4a7; HNF4a8; HNF4a9; HNF4alpha; HNF4alpha10/11/12; MODY; MODY1; NR2A1; NR2A21; nuclear receptor subfamily 2 group A member 1; TCF; TCF14; TCF-14; transcription factor 14; transcription factor HNF-4
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_849180.1; NP_000448.3; NP_849181.1. Purification: Antigen affinity purified. Names and symbols: HNF4A; TCF; HNF4; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; hepatocyte nuclear factor 4, alpha; HNF4-alpha; transcription factor-14; FLJ39654; HNF4alpha; MODY; MODY1; HNF4alpha10/11/12; hepatic nuclear factor 4 alpha
  • Reactivity Supplier
    Human, Dog, Pig
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HNF-4-alpha using anti-HNF-4-alpha antibody (M00179-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HNF-4-alpha antigen affinity purified monoclonal antibody (Catalog # M00179-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for HNF-4-alpha at approximately 53 kDa. The expected band size for HNF-4-alpha is at 53 kDa.
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