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Western Blot analysis of HT29 cells using HNF-4alpha Polyclonal Antibody
Western Blot analysis of HT29 cells using HNF-4alpha Polyclonal Antibody
Western Blot analysis of HT29 cells using HNF-4alpha Polyclonal Antibody

HNF4A Antibody

CSB-PA002939
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHNF4A
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Overview

  • Supplier
    Cusabio
  • Product Name
    HNF4A Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3172
  • Target name
    HNF4A
  • Target description
    hepatocyte nuclear factor 4 alpha
  • Target synonyms
    FRTS4, HNF4, HNF4a7, HNF4a8, HNF4a9, HNF4alpha, MODY, MODY1, NR2A1, NR2A21, TCF, TCF-14, TCF14, hepatocyte nuclear factor 4-alpha, HNF4A wild type, HNF4alpha10/11/12, hepatic nuclear factor 4 alpha, nuclear receptor subfamily 2 group A member 1, transcription factor 14, transcription factor HNF-4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP41235
  • Protein Name
    Hepatocyte nuclear factor 4-alpha
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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BRL-3A cell lysates probed with HNF4A Polyclonal Antibody, Unconjugated (bs-3828R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Product group Antibodies
References
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Figure 1. Western blot analysis of HNF-4-alpha using anti-HNF-4-alpha antibody (M00179-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HNF-4-alpha antigen affinity purified monoclonal antibody (Catalog # M00179-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for HNF-4-alpha at approximately 53 kDa. The expected band size for HNF-4-alpha is at 53 kDa.
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