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Anti-HSD11B1 Antibody

Anti-HSD11B1 Antibody

A95458
Antibodies.com
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-HSD11B1 Antibody
  • Delivery Days Customer
    7
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Rabbit polyclonal antibody to HSD11B1.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HSD11B1 using anti-HSD11B1 antibody (A01565). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human HCCP tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD11B1 antigen affinity purified polyclonal antibody (Catalog # A01565) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD11B1 at approximately 36 kDa. The expected band size for HSD11B1 is at 32 kDa.
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This image was kindly submitted by Dragos Scarlet DVM, MSc from the University of Vienna as part of the Bioss Discovery Progarm.\n\nParaffin embedded horse duodenum labeled with Anti-11ßHSD1 Polyclonal Antibody, Unconjugated (bs-3904R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
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