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Western Blot analysis of K562 cells using 11beta-HSD1 Polyclonal Antibody
Western Blot analysis of K562 cells using 11beta-HSD1 Polyclonal Antibody
Western Blot analysis of K562 cells using 11beta-HSD1 Polyclonal Antibody

HSD11B1 Antibody

CSB-PA006123
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHSD11B1
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Overview

  • Supplier
    Cusabio
  • Product Name
    HSD11B1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3290
  • Target name
    HSD11B1
  • Target description
    hydroxysteroid 11-beta dehydrogenase 1
  • Target synonyms
    11-DH, 11-beta-HSD1, CORTRD2, HDL, HSD11, HSD11B, HSD11L, SDR26C1, 11-beta-hydroxysteroid dehydrogenase 1, 7-oxosteroid reductase, corticosteroid 11-beta-dehydrogenase isozyme 1, short chain dehydrogenase/reductase family 26C member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP28845
  • Protein Name
    Corticosteroid 11-beta-dehydrogenase isozyme 1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HSD11B1 using anti-HSD11B1 antibody (A01565). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human HCCP tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD11B1 antigen affinity purified polyclonal antibody (Catalog # A01565) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD11B1 at approximately 36 kDa. The expected band size for HSD11B1 is at 32 kDa.
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