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This image was kindly submitted by Dragos Scarlet DVM, MSc from the University of Vienna as part of the Bioss Discovery Progarm.\n\nParaffin embedded horse duodenum labeled with Anti-11ßHSD1 Polyclonal Antibody, Unconjugated (bs-3904R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
This image was kindly submitted by Dragos Scarlet DVM, MSc from the University of Vienna as part of the Bioss Discovery Progarm.\n\nParaffin embedded horse duodenum labeled with Anti-11ßHSD1 Polyclonal Antibody, Unconjugated (bs-3904R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
This image was kindly submitted by Dragos Scarlet DVM, MSc from the University of Vienna as part of the Bioss Discovery Progarm.\n\nParaffin embedded horse duodenum labeled with Anti-11ßHSD1 Polyclonal Antibody, Unconjugated (bs-3904R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

HSD11B1 Polyclonal Antibody

BS-3904R
Bioss Antibodies
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetHSD11B1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    HSD11B1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID3290
  • Target name
    HSD11B1
  • Target description
    hydroxysteroid 11-beta dehydrogenase 1
  • Target synonyms
    11-beta-HSD1; 11-DH; corticosteroid 11-beta-dehydrogenase isozyme 1; CORTRD2; HDL; HSD11; HSD11B; HSD11L; SDR26C1; short chain dehydrogenase/reductase family 26C member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP28845
  • Protein Name
    Corticosteroid 11-beta-dehydrogenase isozyme 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • The expression of 11beta-hydroxysteroid dehydrogenase type 1 is increased in experimental periodontitis in rats. Nakata T et al., 2016 Oct 3, BMC Oral Health
    Read more
  • Preeclampsia is associated with a deficiency of lipoxin A4, an endogenous anti-inflammatory mediator. Xu Z et al., 2014 Jul, Fertil Steril
    Read more

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Figure 1. Western blot analysis of HSD11B1 using anti-HSD11B1 antibody (A01565). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human HCCP tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD11B1 antigen affinity purified polyclonal antibody (Catalog # A01565) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD11B1 at approximately 36 kDa. The expected band size for HSD11B1 is at 32 kDa.
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