ProductsBack/HSD11B1 Polyclonal Antibody

HSD11B1 Polyclonal Antibody

CAC13769

ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry

Product group Antibodies

TargetHSD11B1

Overview

Supplier
Biomatik
Product Name
HSD11B1 Polyclonal Antibody
Delivery Days Customer
12
Applications
ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Certification
Research Use Only
Clonality
Polyclonal
Conjugate
Unconjugated
Gene ID3290
Target name
HSD11B1
Target description
hydroxysteroid 11-beta dehydrogenase 1
Target synonyms
11-beta-HSD1; 11-DH; corticosteroid 11-beta-dehydrogenase isozyme 1; CORTRD2; HDL; HSD11; HSD11B; HSD11L; SDR26C1; short chain dehydrogenase/reductase family 26C member 1
Host
Rabbit
Isotype
IgG
Protein IDP28845
Protein Name
Corticosteroid 11-beta-dehydrogenase isozyme 1
Scientific Description
The HSD11B1 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC, IF.
Storage Instruction
-20°C,2°C to 8°C
UNSPSC
12352203

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Figure 1. Western blot analysis of HSD11B1 using anti-HSD11B1 antibody (A01565). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human HCCP tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD11B1 antigen affinity purified polyclonal antibody (Catalog # A01565) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD11B1 at approximately 36 kDa. The expected band size for HSD11B1 is at 32 kDa.

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$This image was kindly submitted by Dragos Scarlet DVM, MSc from the University of Vienna as part of the Bioss Discovery Progarm.\n\nParaffin embedded horse duodenum labeled with Anti-11ßHSD1 Polyclonal Antibody, Unconjugated (bs-3904R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$