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Anti-HSD3B1 Antibody

144-66533
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetHSD3B1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-HSD3B1 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Gene ID3283
  • Target name
    HSD3B1
  • Target description
    hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
  • Target synonyms
    3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I; 3BETAHSD; 3-beta-HSD I; 3-beta-hydroxy-5-ene steroid dehydrogenase; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; 3-beta-hydroxysteroid 3-dehydrogenase; delta-5-3-ketosteroid isomerase; dihydrotestosterone oxidoreductase; HSD3B; HSDB3; HSDB3A; progesterone reductase; SDR11E1; short chain dehydrogenase/reductase family 11E, member 1; steroid Delta-isomerase; trophoblast antigen FDO161G
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    HSD3B1 pAb
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HSD3B1 using anti-HSD3B1 antibody (A02856-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human MDA-MB-453 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD3B1 antigen affinity purified polyclonal antibody (Catalog # A02856-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD3B1 at approximately 42KD. The expected band size for HSD3B1 is at 42KD.
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