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Western Blot Positive WB detected in: Human placenta tissue, HEK293 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate All lanes: HSD3B1 antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 43 kDa Observed band size: 43 kDa
Western Blot Positive WB detected in: Human placenta tissue, HEK293 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate All lanes: HSD3B1 antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 43 kDa Observed band size: 43 kDa
Western Blot Positive WB detected in: Human placenta tissue, HEK293 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate All lanes: HSD3B1 antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 43 kDa Observed band size: 43 kDa

HSD3B1 Antibody

CSB-PA010781LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetHSD3B1
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Overview

  • Supplier
    Cusabio
  • Product Name
    HSD3B1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3283
  • Target name
    HSD3B1
  • Target description
    hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
  • Target synonyms
    3BETAHSD, HSD3B, HSDB3, HSDB3A, SDR11E1, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I, 3-beta-HSD I, 3-beta-hydroxy-5-ene steroid dehydrogenase, 3-beta-hydroxy-Delta(5)-steroid dehydrogenase, 3-beta-hydroxysteroid 3-dehydrogenase, delta-5-3-ketosteroid isomerase, dihydrotestosterone oxidoreductase, progesterone reductase, short chain dehydrogenase/reductase family 11E, member 1, steroid Delta-isomerase, trophoblast antigen FDO161G
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP14060
  • Protein Name
    3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
  • Scientific Description
    3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. Efficiently catalyzes the transformation of pregnenolone to progesterone, 17-alpha-hydroxypregnenolone to 17-alpha-hydroxyprogesterone, DHEA to 4-androstenedione, dihydrotestosterone to 5-alpha-androstane-3 beta,17 beta-diol, dehydroepiandrosterone to androstenedione and 5-alpha-androstan-3 beta,17 beta-diol to 5-alpha-dihydrotestosterone.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HSD3B1 using anti-HSD3B1 antibody (A02856-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human MDA-MB-453 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD3B1 antigen affinity purified polyclonal antibody (Catalog # A02856-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD3B1 at approximately 42KD. The expected band size for HSD3B1 is at 42KD.
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