Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with HSD3B1 antibody [N1C1] (GTX114081) diluted at 1:500 and competitor's antibody (sc-30820) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![HSD3B1 antibody [N1C1] detects HSD3B1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. HSD3B1 stained by HSD3B1 antibody [N1C1] (GTX114081) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX114081/GTX114081_40436_20181207_IHC-P_w_23060501_628.webp "HSD3B1 antibody [N1C1] detects HSD3B1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. HSD3B1 stained by HSD3B1 antibody [N1C1] (GTX114081) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with HSD3B1 antibody [N1C1] (GTX114081) diluted at 1:500 and competitor's antibody (sc-30820) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
HSD3B1 antibody [N1C1]
GTX114081
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetHSD3B1
Overview
- SupplierGeneTex
- Product NameHSD3B1 antibody [N1C1]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.67 mg/ml
- ConjugateUnconjugated
- Gene ID3283
- Target nameHSD3B1
- Target descriptionhydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
- Target synonyms3BETAHSD, HSD3B, HSDB3, HSDB3A, SDR11E1, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I, 3-beta-HSD I, 3-beta-hydroxy-5-ene steroid dehydrogenase, 3-beta-hydroxy-Delta(5)-steroid dehydrogenase, 3-beta-hydroxysteroid 3-dehydrogenase, delta-5-3-ketosteroid isomerase, dihydrotestosterone oxidoreductase, progesterone reductase, short chain dehydrogenase/reductase family 11E, member 1, steroid Delta-isomerase, trophoblast antigen FDO161G
- HostRabbit
- IsotypeIgG
- Scientific Description3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. Efficiently catalyzes the transformation of pregnenolone to progesterone, 17-alpha-hydroxypregnenolone to 17-alpha-hydroxyprogesterone, DHEA to 4-androstenedione, dihydrotestosterone to 5-alpha-androstane-3 beta,17 beta-diol, dehydroepiandrosterone to androstenedione and 5-alpha-androstan-3 beta,17 beta-diol to 5-alpha-dihydrotestosterone.
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Kotlarczyk AM, Grzyb M, Korzekwa AJ. Regulation of uterine function during estrous cycle, anestrus phase and pregnancy by steroids in red deer (Cervus elaphus L.). Sci Rep. 2021,11(1):20109. doi: 10.1038/s41598-021-99601-5Read this paper
Datasheet
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