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Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in zona fasciculata.
Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in zona fasciculata.
Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in zona fasciculata.

Anti-HSD3B1 Antibody

HPA044028
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetHSD3B1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-HSD3B1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3283
  • Target name
    HSD3B1
  • Target description
    hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
  • Target synonyms
    3BETAHSD, HSD3B, HSDB3, HSDB3A, SDR11E1, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I, 3-beta-HSD I, 3-beta-hydroxy-5-ene steroid dehydrogenase, 3-beta-hydroxy-Delta(5)-steroid dehydrogenase, 3-beta-hydroxysteroid 3-dehydrogenase, delta-5-3-ketosteroid isomerase, dihydrotestosterone oxidoreductase, progesterone reductase, short chain dehydrogenase/reductase family 11E, member 1, steroid Delta-isomerase, trophoblast antigen FDO161G
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP14060
  • Protein Name
    3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of HSD3B1 using anti-HSD3B1 antibody (A02856-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human MDA-MB-453 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD3B1 antigen affinity purified polyclonal antibody (Catalog # A02856-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD3B1 at approximately 42KD. The expected band size for HSD3B1 is at 42KD.
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