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ProductsBack/Anti-Hu CD36 Biotin

Anti-Hu CD36 Biotin

1B-451
EXBIO
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityHuman
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Overview

  • Supplier
    EXBIO
  • Product Name
    Anti-Hu CD36 Biotin
  • Delivery Days Customer
    7
  • Application Supplier Note
    Flow cytometry: Recommended dilution: 1 microg/ml.
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clone ID
    TR9
  • Concentration
    1 mg/ml
  • Conjugate
    Biotin
  • Isotype
    IgG1
  • Scientific Description
    CD36 (fatty acid translocase, FAT) is an 88 kDa ditopic glycosylated protein that belongs to the class B family of scavenger receptors. CD36 is expressed by most resting marginal zone B cells but not by follicular and B1 B cells, and it is rapidly induced on follicular B cells in vitro upon TLR and CD40 stimulation. CD36 does not affect the development of B cells, but modulates both primary and secondary antibody response. Similarly to glucose transporter GLUT4, CD36 is translocated from intracellular pools to the plasma membrane following cell stimulation by insulin. In mouse, CD36 is responsible for gustatory perception of long-chain fatty acids.
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD36 using anti-CD36 antibody (A01189-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD36 antigen affinity purified polyclonal antibody (Catalog # A01189-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD36 at approximately 88 kDa. The expected band size for CD36 is at 53 kDa.
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