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Anti-human Perforin mAb (Pf-344), biotin

Anti-human Perforin mAb (Pf-344), biotin

3465-6
Mabtech
ApplicationsFlow Cytometry, Western Blot, ELISA, ELISpot Assay, ImmunoCytoChemistry
Product group Antibodies
TargetPRF1
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Overview

  • Supplier
    Mabtech
  • Product Name
    Anti-human Perforin mAb (Pf-344), biotin
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, Western Blot, ELISA, ELISpot Assay, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    Pf-344
  • Conjugate
    Biotin
  • Gene ID5551
  • Target name
    PRF1
  • Target description
    perforin 1
  • Target synonyms
    HPLH2, P1, PFP, perforin-1, cytolysin, lymphocyte pore-forming protein, perforin 1 (pore forming protein)
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    Validated for our assays. - This monoclonal antibody enables specific detection of human perforin in immunoassays such as ELISpot, FluoroSpot, ELISA, flow cytometry, and Western blot.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Perforin using anti-Perforin antibody (PB9155). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human Perforin protein 0.5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Perforin antigen affinity purified polyclonal antibody (Catalog # PB9155) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Perforin at approximately 40 kDa. The expected band size for Perforin is at 40 kDa.
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