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Anti-Integrin alpha-1 [AQC2], Human IgG1, kappa

AB04544-10.0
Absolute Antibody
ApplicationsWestern Blot, ELISA, Other Application
Product group Antibodies
TargetITGA1
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Integrin alpha-1 [AQC2], Human IgG1, kappa
  • Delivery Days Customer
    9
  • Application Supplier Note
    The structure of the humanized version of this antibody was determined using x-ray crystallography. The humanized antibody was used for an ELISA on human alpha 1 beta 1 I domain (Karpusas et al., 2003; PMID:12662928). This antibody was used for a western blot on alpha-1 integrin of different animals, showing that this antibody could also bind sheep alpha-1 integrin. This antibody was used as an aerosol to prevent allergic reactions in the lungs of sheep. It only showed an effect on the late airway responses and no effect on the initial allergic reaction (Abraham et al., 2004; PMID:14578216).
  • Applications
    Western Blot, ELISA, Other Application
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    AQC2
  • Gene ID3672
  • Target name
    ITGA1
  • Target description
    integrin subunit alpha 1
  • Target synonyms
    CD49 antigen-like family member A; CD49a; integrin alpha-1; laminin and collagen receptor; very late activation protein 1; very late antigen-1; VLA1; VLA-1
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDP56199
  • Protein Name
    Integrin alpha-1
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Integrin Alpha 1/ITGA1 using anti-Integrin Alpha 1/ITGA1 antibody (PA1045-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin Alpha 1/ITGA1 antigen affinity purified polyclonal antibody (Catalog # PA1045-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin Alpha 1/ITGA1 at approximately 190-200 kDa. The expected band size for Integrin Alpha 1/ITGA1 is at 190-200 kDa.
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