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ProductsBack/ITGA1/CD49a/Integrin Alpha 1 Antibody (clone TS2/7)

ITGA1/CD49a/Integrin Alpha 1 Antibody (clone TS2/7)

LS-C783537
LifeSpan BioSciences
ApplicationsFlow Cytometry
Product group Antibodies
TargetITGA1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ITGA1/CD49a/Integrin Alpha 1 Antibody (clone TS2/7)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.5 microg per million cells in 100 microl volume. It is recommended that the reagent be titrated for optimal performance for each application. Additional reported applications include: immunoprecipitation and immunohistochemical staining of acetone-fixed frozen tissue sections. Flo (0.5 µg/10E6 cells) Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. Additional reported applications include: immunoprecipitation and immunohistochemical staining of acetone-fixed frozen tissue sections
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    TS2/7
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3672
  • Target name
    ITGA1
  • Target description
    integrin subunit alpha 1
  • Target synonyms
    CD49 antigen-like family member A; CD49a; integrin alpha-1; laminin and collagen receptor; very late activation protein 1; very late antigen-1; VLA1; VLA-1
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Integrin Alpha 1/ITGA1 using anti-Integrin Alpha 1/ITGA1 antibody (PA1045-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin Alpha 1/ITGA1 antigen affinity purified polyclonal antibody (Catalog # PA1045-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin Alpha 1/ITGA1 at approximately 190-200 kDa. The expected band size for Integrin Alpha 1/ITGA1 is at 190-200 kDa.
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