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ProductsBack/Goat anti-ITGA1 / VLA1

Goat anti-ITGA1 / VLA1

EB12558
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetITGA1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-ITGA1 / VLA1 Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID3672
  • Target name
    ITGA1
  • Target description
    integrin subunit alpha 1
  • Target synonyms
    CD49a, VLA1, integrin alpha-1, CD49 antigen-like family member A, VLA-1, laminin and collagen receptor, very late activation protein 1, very late antigen-1
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_852478.1. Purification: Antigen affinity purified. Names and symbols: ITGA1; integrin, alpha 1; CD49a; VLA1; CD49 antigen-like family member A; VLA-1; integrin alpha-1; laminin and collagen receptor; very late activation protein 1
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Integrin Alpha 1/ITGA1 using anti-Integrin Alpha 1/ITGA1 antibody (PA1045-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin Alpha 1/ITGA1 antigen affinity purified polyclonal antibody (Catalog # PA1045-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin Alpha 1/ITGA1 at approximately 190-200 kDa. The expected band size for Integrin Alpha 1/ITGA1 is at 190-200 kDa.
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