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Human U937 cells probed with Integrin alpha 1 Polyclonal Antibody, Unconjugated (bs-2095R) at 0.2ug for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Human U937 cells probed with Integrin alpha 1 Polyclonal Antibody, Unconjugated (bs-2095R) at 0.2ug for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Human U937 cells probed with Integrin alpha 1 Polyclonal Antibody, Unconjugated (bs-2095R) at 0.2ug for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Integrin alpha 1 Polyclonal Antibody

BS-2095R
Bioss
ApplicationsWestern Blot
Product group Antibodies
ReactivityBovine, Equine, Human, Mouse, Rat
TargetITGA1
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Overview

  • Supplier
    Bioss
  • Product Name
    Integrin alpha 1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Applications Supplier
    WB(1:500-2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID3672
  • Target name
    ITGA1
  • Target description
    integrin subunit alpha 1
  • Target synonyms
    CD49a, VLA1, integrin alpha-1, CD49 antigen-like family member A, VLA-1, laminin and collagen receptor, very late activation protein 1, very late antigen-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP56199
  • Protein Name
    Integrin alpha-1
  • Reactivity
    Bovine, Equine, Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

Datasheet

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Figure 1. Western blot analysis of Integrin Alpha 1/ITGA1 using anti-Integrin Alpha 1/ITGA1 antibody (PA1045-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin Alpha 1/ITGA1 antigen affinity purified polyclonal antibody (Catalog # PA1045-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin Alpha 1/ITGA1 at approximately 190-200 kDa. The expected band size for Integrin Alpha 1/ITGA1 is at 190-200 kDa.
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