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Anti-IRF4 Antibody

AMAB91979
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetIRF4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-IRF4 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL14615
  • Conjugate
    Unconjugated
  • Gene ID3662
  • Target name
    IRF4
  • Target description
    interferon regulatory factor 4
  • Target synonyms
    IMD131, LSIRF, MUM1, NF-EM5, SHEP8, interferon regulatory factor 4, lymphocyte-specific interferon regulatory factor, multiple myeloma oncogene 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ15306
  • Protein Name
    Interferon regulatory factor 4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MUM1/IRF4 using anti-MUM1/IRF4 antibody (A00401-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hut-78 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MUM1/IRF4 antigen affinity purified polyclonal antibody (Catalog # A00401-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MUM1/IRF4 at approximately 60 kDa. The expected band size for MUM1/IRF4 is at 52 kDa.
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