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Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IRF4 / MUM1 antibody (GTX129135) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IRF4 / MUM1 antibody (GTX129135) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IRF4 / MUM1 antibody (GTX129135) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

IRF4 / MUM1 antibody

GTX129135
GeneTex
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHuman
TargetIRF4
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Overview

  • Supplier
    GeneTex
  • Product Name
    IRF4 / MUM1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3662
  • Target name
    IRF4
  • Target description
    interferon regulatory factor 4
  • Target synonyms
    IMD131, LSIRF, MUM1, NF-EM5, SHEP8, interferon regulatory factor 4, lymphocyte-specific interferon regulatory factor, multiple myeloma oncogene 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    The protein encoded by this gene belongs to the IRF (interferon regulatory factor) family of transcription factors, characterized by an unique tryptophan pentad repeat DNA-binding domain. The IRFs are important in the regulation of interferons in response to infection by virus, and in the regulation of interferon-inducible genes. This family member is lymphocyte specific and negatively regulates Toll-like-receptor (TLR) signaling that is central to the activation of innate and adaptive immune systems. A chromosomal translocation involving this gene and the IgH locus, t(6;14)(p25;q32), may be a cause of multiple myeloma. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Fucci C, Resnati M, Riva E, et al. The Interaction of the Tumor Suppressor FAM46C with p62 and FNDC3 Proteins Integrates Protein and Secretory Homeostasis. Cell Rep. 2020,32(12):108162. doi: 10.1016/j.celrep.2020.108162
    Read this paper

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Figure 1. Western blot analysis of MUM1/IRF4 using anti-MUM1/IRF4 antibody (A00401-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hut-78 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MUM1/IRF4 antigen affinity purified polyclonal antibody (Catalog # A00401-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MUM1/IRF4 at approximately 60 kDa. The expected band size for MUM1/IRF4 is at 52 kDa.
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