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ProductsBack/IRF4 Recombinant Antibody
Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MUM1 Monoclonal Antibody, Unconjugated (bsm-54718R) at 1:50 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MUM1 Monoclonal Antibody, Unconjugated (bsm-54718R) at 1:50 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MUM1 Monoclonal Antibody, Unconjugated (bsm-54718R) at 1:50 for 30 minutes at room temperature, DAB staining.

IRF4 Recombinant Antibody

bsm-54718R
Bioss Antibodies
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetIRF4
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    MUM1 Monoclonal Antibody
  • Delivery Days Customer
    7
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(WB=1:500-2000), IHC-P(IHC-P=1:50-200), IHC-F(IHC-F=1:50-200), IP(IP=1:20-50), IF(IF=1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    6H0
  • Conjugate
    Unconjugated
  • Gene ID3662
  • Target name
    IRF4
  • Target description
    interferon regulatory factor 4
  • Target synonyms
    interferon regulatory factor 4; LSIRF; lymphocyte-specific interferon regulatory factor; multiple myeloma oncogene 1; MUM1; NF-EM5; SHEP8
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MUM1/IRF4 using anti-MUM1/IRF4 antibody (A00401-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hut-78 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MUM1/IRF4 antigen affinity purified polyclonal antibody (Catalog # A00401-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MUM1/IRF4 at approximately 60 kDa. The expected band size for MUM1/IRF4 is at 52 kDa.
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