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Immunohistochemical staining of human esophagus shows moderate cytoplasmic positivity in squamous epithelial cells.
Immunohistochemical staining of human esophagus shows moderate cytoplasmic positivity in squamous epithelial cells.
Immunohistochemical staining of human esophagus shows moderate cytoplasmic positivity in squamous epithelial cells.

Anti-ITPKC Antibody

HPA050760
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetITPKC
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ITPKC
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID80271
  • Target name
    ITPKC
  • Target description
    inositol-trisphosphate 3-kinase C
  • Target synonyms
    IP3-3KC, IP3KC, inositol-trisphosphate 3-kinase C, IP3 3-kinase C, IP3K C, InsP 3 kinase C, inositol 1,4,5-trisphosphate 3-kinase C, insP 3-kinase C
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96DU7
  • Protein Name
    Inositol-trisphosphate 3-kinase C
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ITPKC using anti-ITPKC antibody (A07845-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITPKC antigen affinity purified polyclonal antibody (Catalog # A07845-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITPKC at approximately 100 kDa. The expected band size for ITPKC is at 75 kDa.
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