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Immunohistochemical staining of human liver shows strong granular positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong granular positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong granular positivity in hepatocytes.

Anti-JADE1 Antibody

HPA020016
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetJADE1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-JADE1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID79960
  • Target name
    JADE1
  • Target description
    jade family PHD finger 1
  • Target synonyms
    PHF17, protein Jade-1, PHD finger protein 17, PHD protein Jade-1, gene for apoptosis and differentiation in epithelia, jade family PHD finger protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6IE81
  • Protein Name
    Protein Jade-1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of PHF17/JADE1 using anti-PHF17/JADE1 antibody (A07611-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHF17/JADE1 antigen affinity purified polyclonal antibody (Catalog # A07611-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PHF17/JADE1 at approximately 96 kDa. The expected band size for PHF17/JADE1 is at 96 kDa.
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