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WB analysis of various sample lysates using GTX32786 PHF17 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX32786 PHF17 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX32786 PHF17 antibody. Dilution : 1:1000 Loading : 25microg per lane

PHF17 antibody

GTX32786
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetJADE1
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Overview

  • Supplier
    GeneTex
  • Product Name
    PHF17 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:10 - 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID79960
  • Target name
    JADE1
  • Target description
    jade family PHD finger 1
  • Target synonyms
    PHF17, protein Jade-1, PHD finger protein 17, PHD protein Jade-1, gene for apoptosis and differentiation in epithelia, jade family PHD finger protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6IE81
  • Protein Name
    Protein Jade-1
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PHF17/JADE1 using anti-PHF17/JADE1 antibody (A07611-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHF17/JADE1 antigen affinity purified polyclonal antibody (Catalog # A07611-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PHF17/JADE1 at approximately 96 kDa. The expected band size for PHF17/JADE1 is at 96 kDa.
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