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Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA743563ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA743563ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA743563ESR1HU at dilution of 1:100

JADE1 Antibody

CSB-PA743563ESR1HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetJADE1
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Overview

  • Supplier
    Cusabio
  • Product Name
    JADE1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID79960
  • Target name
    JADE1
  • Target description
    jade family PHD finger 1
  • Target synonyms
    gene for apoptosis and differentiation in epithelia; jade family PHD finger protein 1; PHD finger protein 17; PHD protein Jade-1; PHF17; protein Jade-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6IE81
  • Protein Name
    Protein Jade-1
  • Scientific Description
    Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Transcriptional coactivator, it may also promote acetylation of nucleosomal histone H4 by KAT5. Promotes apoptosis. May act as a renal tumor suppressor.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PHF17/JADE1 using anti-PHF17/JADE1 antibody (A07611-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHF17/JADE1 antigen affinity purified polyclonal antibody (Catalog # A07611-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PHF17/JADE1 at approximately 96 kDa. The expected band size for PHF17/JADE1 is at 96 kDa.
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