Anti-Kinetochore protein NDC80 homolog [Hec1 (9G3)]

Absolute Antibody

Anti-Kinetochore protein NDC80 homolog [Hec1 (9G3)]

9

This antibody specifically binds amino acids 200-215 in the N terminal globular domain of the human Hec1 protein, exterior to Spc24 at metaphase kinetochores. It binds in a region which resides within the well-ordered calponin homology domain of the prote

This antibody is widely used in studies involving the role of Hec1 protein in mitosis. This antibody can immunoprecipitate human Hec1 protein from nuclear, cytoplasmic, membrane and other components of T24 human bladder carcinoma cells. This antibody was capable of recognizing a 76-KDa protein in western blot. It was reported that when T24 human bladder carcinoma cells were synchronized in S phase and microinjected with this antibody the cells contained multiple, fragmented nuclei (PMID: 9315664). Preincubation of the antibody with C-2 peptide (comprising amino acids 200-215 of Hec1) prevented the antibody from recognizing kinetochores in immunofluorescence assays. Immunofluorescent staining of HeLa and PtK1 cells in metaphase with 9G3 and another anti-Spc24 antibody revealed that the N terminus of Hec1 was always exterior to Spc24 at kinetochores of bi-oriented chromosomes. It was further reported that PtK1 cells injected with this antibody exhibited mitotic defects (PMID: 17129782). A recombinantly generated version of this antibody rMAb-Hec1 robustly recognized kinetochores however, the original antibody exhibited more spindle staining and overall background staining when compared to the recombinant version (PMID: 34970967).

ImmunoFluorescence, ImmunoPrecipitation, Western Blot

Research Use Only

Monoclonal

Hec1 (9G3)

NDC80

NDC80 kinetochore complex component

HEC; HEC1; highly expressed in cancer protein; highly expressed in cancer, rich in leucine heptad repeats; HsHec1; hsNDC80; kinetochore associated 2; kinetochore protein Hec1; kinetochore protein NDC80 homolog; kinetochore-associated protein 2; KNTC2; NDC80 homolog, kinetochore complex component; NDC80 kinetochore complex component homolog; retinoblastoma-associated protein HEC; TID3

Mouse

IgG1

Kinetochore protein NDC80 homolog

Human

-20°C,2°C to 8°C

12352203